The race will start in the Scottish borders in Kelso, which has twice hosted the race before, and will this year stage the start and finish of stage one.
Stage two will begin in Darlington and finish in Redcar, before moving to South Yorkshire for the first time since 2007, where Sheffield will host the start of stage three to Barnsley.
Details of the fourth leg have not yet been confirmed, but stage five will start and finish in Northampton before the finale in Suffolk from Lowestoft to Felixstowe.
“We’re enormously thankful to the local partners who are supporting the delivery of this year’s race, as we look to build on the momentum of June’s successful Lloyds Bank Tour of Britain Women,” said British Cycling events managing director Jonathan Day.
“I’ve no doubt that their communities will be out in force to welcome the riders, and make it another memorable week of racing to cap off a huge summer of cycling.”
The men’s race follows the Tour of Britain Women which returned in June, also organised by British Cycling, after it was cancelled in 2023.